Reunala T, Brummer-Korvenkontio H, Räsänen L, François G, Palosuo T
Department of Dermatology, Helsinki University Hospital, Finland.
J Allergy Clin Immunol. 1994 Nov;94(5):902-6. doi: 10.1016/0091-6749(94)90158-9.
Mosquito bites frequently cause cutaneous wheal and flare reactions, and recent immunoblotting studies have shown specific anti-saliva IgE antibodies in many persons who have such reactions.
The study was designed to show that human serum containing mosquito saliva-specific IgE antibodies can produce histamine release in vitro and whealing in vivo.
Two mosquito bite-tolerant subjects had bite challenges and Prausnitz-Küstner tests with heated and unheated serum from one patient with Aedes mosquito allergy. Immunoblotting and basophil histamine release tests were performed with the patient's and subjects' sera.
Both mosquito bite-tolerant subjects had positive Prausnitz-Küstner reactions, which indicated a successful transfer of cutaneous mosquito hypersensitivity. The ordinary and passive basophil histamine release tests also produced positive results with Aedes communis antigens.
The results of the Prausnitz-Küstner test, immunoblotting, and basophil histamine release tests are consistent with the hypothesis that mosquito bite whealing is mediated by specific anti-saliva IgE antibodies.
蚊虫叮咬常引起皮肤风团和潮红反应，最近的免疫印迹研究表明，许多有此类反应的人存在特异性抗唾液IgE抗体。
本研究旨在表明，含有蚊虫唾液特异性IgE抗体的人血清可在体外诱导组胺释放，并在体内引发风团。
两名对蚊虫叮咬耐受的受试者接受了叮咬激发试验，并与一名对伊蚊过敏患者的加热和未加热血清进行了普劳斯尼茨-屈斯特纳试验。对患者和受试者的血清进行了免疫印迹和嗜碱性粒细胞组胺释放试验。
两名对蚊虫叮咬耐受的受试者普劳斯尼茨-屈斯特纳反应均呈阳性，这表明皮肤蚊虫超敏反应成功转移。普通和被动嗜碱性粒细胞组胺释放试验对普通伊蚊抗原也产生了阳性结果。
普劳斯尼茨-屈斯特纳试验、免疫印迹和嗜碱性粒细胞组胺释放试验的结果与蚊虫叮咬风团由特异性抗唾液IgE抗体介导的假说一致。
