Ravi S K, Naidu M U, Sekhar E C, Rao T R, Shobha J C, Rani P U, Surya K J
Department of Clinical Pharmacology and Therapeutics, Nizam's Institute of Medical Sciences, Panjagutta, Hyderabad, India.
J Chromatogr B Biomed Sci Appl. 1997 Mar 28;691(1):208-11. doi: 10.1016/s0378-4347(96)00419-7.
A rapid, reproducible high-performance liquid chromatographic method for the determination of secnidazole, 5-nitroimidazole class of antiprotozoals from blood is described. Metronidazole was used as an internal standard. A simple extraction step with dichloromethane was done before chromatography on a C18 column with the wavelength fixed at 276 nm on the UV detector. Blood levels up to 500 ng/ml have been measured with good precision in the healthy volunteers after 1 g of secnidazole was administered. The present described method can readily be utilized for routine pharmacokinetic studies.
本文描述了一种快速、可重现的高效液相色谱法，用于测定血液中的塞克硝唑（一种5-硝基咪唑类抗原虫药物）。甲硝唑用作内标。在C18柱上进行色谱分析之前，先用二氯甲烷进行简单萃取，紫外检测器波长固定在276 nm。在健康志愿者服用1 g塞克硝唑后，已精确测定出血药浓度高达500 ng/ml。本文所述方法可很容易地用于常规药代动力学研究。
