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在一例因头部被猫抓伤后发生的多杀巴斯德菌脑膜炎病例中应用随机扩增多态性DNA技术

Use of random amplification of polymorphic DNA in a case of Pasteurella multocida meningitis that occurred following a cat scratch on the head.

作者信息

Schuur P M, Haring A J, van Belkum A, Draaisma J M, Buiting A G

机构信息

Department of Medical Microbiology, St. Elisabeth Hospital, Tilburg, The Netherlands.

出版信息

Clin Infect Dis. 1997 May;24(5):1004-6. doi: 10.1093/clinids/24.5.1004.

Abstract

We cultured Pasteurella multocida from the cerebrospinal fluid (CSF) of a 4-month-old infant who presented with meningitis. The patient had been scratched on the head by a cat. Culture of the cat's claws also yielded P. multocida. The isolates had identical biochemical patterns. Analysis of both strains by random amplification of polymorphic DNA and comparison of these strains with P. multocida strains isolated from other cats showed that the two strains were identical and completely different from the unrelated isolates. Our patient's meningitis most likely resulted from direct inoculation of P. multocida into the CSF.

摘要

我们从一名患有脑膜炎的4个月大婴儿的脑脊液(CSF)中培养出多杀巴斯德菌。该患者头部曾被猫抓伤。对猫爪进行培养也分离出多杀巴斯德菌。分离株具有相同的生化模式。通过随机扩增多态性DNA对这两种菌株进行分析,并将这些菌株与从其他猫分离出的多杀巴斯德菌菌株进行比较,结果表明这两种菌株相同,且与无关分离株完全不同。我们这位患者的脑膜炎很可能是由于多杀巴斯德菌直接接种到脑脊液中所致。

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