Loubinoux J, Lozniewski A, Lion C, Garin D, Weber M, Le Faou A E
Laboratoire de Virologie, CHU Nancy-Brabois, 54511 Vandoeuvre-lès-Nancy Cédex, France.
J Clin Microbiol. 1999 Aug;37(8):2488-92. doi: 10.1128/JCM.37.8.2488-2492.1999.
Fifty-six Pasteurella multocida strains (40 P. multocida subsp. septica and 16 P. multocida subsp. multocida strains) isolated from the mouths of 56 dogs among the 134 living in a French canine military training center (132e Groupe Cynophile de l'Armée de Terre, Suippes, France) were studied by use of enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and restriction fragment length polymorphism (RFLP) techniques. Both techniques showed genomic heterogeneity of the strains studied. However, RFLP was more discriminatory than ERIC-PCR for differentiating P. multocida strains. All but three pairs of strains were discriminated by RFLP, suggesting a limited circulation of strains between these dogs living in proximity. Although ERIC-PCR is easier and faster to perform, it cannot be recommended for epidemiological studies of P. multocida strains.
从法国一个犬类军事训练中心(法国陆军第132犬类爱好者团,叙佩斯)的134只犬中选取的56只犬口腔中分离出56株多杀巴斯德菌(40株多杀巴斯德菌败血亚种和16株多杀巴斯德菌多杀亚种菌株),采用肠杆菌基因间重复一致序列聚合酶链反应(ERIC-PCR)和限制性片段长度多态性(RFLP)技术进行研究。两种技术均显示所研究菌株的基因组存在异质性。然而,RFLP在区分多杀巴斯德菌菌株方面比ERIC-PCR更具鉴别力。除三对菌株外,所有菌株均通过RFLP鉴别,这表明生活在附近的这些犬之间菌株的传播有限。尽管ERIC-PCR操作更简便、速度更快,但不推荐用于多杀巴斯德菌菌株的流行病学研究。