Schloter M, Wiehe W, Assmus B, Steindl H, Becke H, Höflich G, Hartmann A
Institute of Soil Ecology, GSF-National Research Center for Environment and Health, Neuherberg, Germany.
Appl Environ Microbiol. 1997 May;63(5):2038-46. doi: 10.1128/aem.63.5.2038-2046.1997.
Monospecific polyclonal antisera raised against Rhizobium leguminosarum bv. trifolii R39, a bacterium which was isolated originally from red clover nodules, were used to study the colonization of roots of leguminous and nonleguminous plants (Pisum sativum, Lupinus albus, Triticúm aestivum, and Zea mays) after inoculation. Eight weeks after inoculation of soil-grown plants, between 0.1 and 1% of the total bacterial population in the rhizospheres of all inoculated plants were identified as R. leguminosarum bv. trifolii R39. To characterize the associative colonization of the nonleguminous plants by R.leguminosarum bv. trifolii R39 in more detail, a time course study was performed with inoculated roots of Z. mays. R. leguminosarum bv. trifolii R39 was found almost exclusively in the rhizosphere soil and on the rhizoplane 4 weeks after inoculation. Colonization of inner root tissues was detected only occasionally at this time. During the process of attachment of R. leguminosarum bv. trifolii R39 to the rhizoplane, bacterial lipopolysaccharides were overexpressed, and this may be important for plant-microbe interaction. Fourteen weeks after inoculation, microcolonies of R. leguminosarum bv. trifolii R39 were detected in lysed cells of the root cortex as well as in intracellular space of central root cylinder cells. At the beginning of flowering (18 weeks after inoculation), the number of R. leguminosarum bv. trifolii R39 organisms decreased in the rhizosphere soil, rhizoplane, and inner root tissue.
针对最初从红三叶草根瘤中分离出的豆科根瘤菌三叶草根瘤菌R39制备的单特异性多克隆抗血清，用于研究接种后豆科植物和非豆科植物（豌豆、白羽扇豆、普通小麦和玉米）根系的定殖情况。对接种土壤种植的植物进行八周后，所有接种植物根际中总细菌群体的0.1%至1%被鉴定为三叶草根瘤菌R39。为了更详细地描述三叶草根瘤菌R39对非豆科植物的联合定殖情况，对接种的玉米根系进行了时间进程研究。接种四周后，三叶草根瘤菌R39几乎只在根际土壤和根表被发现。此时仅偶尔检测到内根组织的定殖情况。在三叶草根瘤菌R39附着到根表的过程中，细菌脂多糖过度表达，这可能对植物-微生物相互作用很重要。接种十四周后，在根皮层的裂解细胞以及中央根柱细胞的细胞内空间中检测到三叶草根瘤菌R39的微菌落。在开花初期（接种十八周后），三叶草根瘤菌R39在根际土壤、根表和内根组织中的数量减少。
