2',3'-环核苷酸3'-磷酸二酯酶：脱髓鞘疾病中的一种新型候选自身抗原。

2',3'-cyclic nucleotide 3'-phosphodiesterase: a novel candidate autoantigen in demyelinating diseases.

作者信息

Rösener M, Muraro P A, Riethmüller A, Kalbus M, Sappler G, Thompson R J, Lichtenfels R, Sommer N, McFarland H F, Martin R

机构信息

Department of Neurology, Eberhard-Karls-University, Tübingen, Germany.

出版信息

J Neuroimmunol. 1997 May;75(1-2):28-34. doi: 10.1016/s0165-5728(96)00230-5.

DOI:10.1016/s0165-5728(96)00230-5

PMID:9143234

Abstract

Autoaggressive T-cells specific for myelin proteins like proteolipid protein (PLP) and myelin basic protein (MBP) are thought to play a major role in the pathogenesis of demyelinating diseases of the central nervous system (CNS). 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) is the third most abundant myelin protein in the CNS. Due to lack of supply with enough CNPase of sufficient purity its immunologic properties have not been studied yet. We subcloned a human CNPase cDNA and expressed human recombinant CNPase (rh-CNPase) in E. coli. Purification of the protein was achieved by Ni(2+)-chelating chromatography. Furthermore we describe for the first time several rh-CNPase specific T-cell lines from a multiple sclerosis patient and a healthy control.

摘要

针对髓鞘蛋白（如蛋白脂质蛋白（PLP）和髓鞘碱性蛋白（MBP））的自身攻击性T细胞被认为在中枢神经系统（CNS）脱髓鞘疾病的发病机制中起主要作用。2',3'-环核苷酸3'-磷酸二酯酶（CNPase）是中枢神经系统中含量第三丰富的髓鞘蛋白。由于缺乏足够纯度的CNPase供应，其免疫学特性尚未得到研究。我们亚克隆了人CNPase cDNA，并在大肠杆菌中表达了人重组CNPase（rh-CNPase）。通过镍（2+）螯合层析实现了该蛋白的纯化。此外，我们首次描述了来自一名多发性硬化症患者和一名健康对照的几种rh-CNPase特异性T细胞系。