Lanza-Jacoby S, Sedkova N, Phetteplace H, Perrotti D
Department of Surgery, Jefferson Medical College.
J Lipid Res. 1997 Apr;38(4):701-10.
Hypertriglyceridemia of sepsis is associated with suppressed tissue lipoprotein lipase (LPL) activities. We investigated the effect of sepsis on lipoprotein lipase gene expression in epididymal fat and soleus muscle from control and septic rats in the fasted and fed state. After 24 h of sepsis, LPL activity decreased significantly in epididymal fat from fasted rats by 45% along with a 57% reduction in LPL mRNA levels and LPL mass. Transcription rate, measured by nuclear run-on assay, decreased by 70% in epididymal fat from fasted septic rats compared to fasted control rats. The synthesis rate of LPL in epididymal fat decreased by 31% while the LPL relative synthesis declined by 50% during sepsis. The turnover rate was not altered. Sepsis in fed rats did not lead to a significant decrease in LPL mRNA in epididymal fat but did lower LPL activity and LPL mass by 45%. The sepsis-induced suppression in soleus LPL activity, LPL mRNA levels, and LPL mass in the fasted state also was observed when septic rats were fed. The results indicate that during fasting sepsis, LPL expression in epididymal fat and possibly soleus muscle involves transcriptional regulation. During sepsis in the fed state, the regulation of LPL in adipose tissue may involve posttranslational mechanisms.
脓毒症的高甘油三酯血症与组织脂蛋白脂肪酶（LPL）活性受抑制有关。我们研究了脓毒症对禁食和进食状态下对照大鼠及脓毒症大鼠附睾脂肪和比目鱼肌中脂蛋白脂肪酶基因表达的影响。脓毒症24小时后，禁食大鼠附睾脂肪中的LPL活性显著降低45%，同时LPL mRNA水平和LPL含量降低57%。通过核转录分析测量的转录率，与禁食对照大鼠相比，禁食脓毒症大鼠附睾脂肪中的转录率降低了70%。脓毒症期间，附睾脂肪中LPL的合成率降低了31%，而LPL相对合成量下降了50%。周转率未改变。进食大鼠的脓毒症并未导致附睾脂肪中LPL mRNA显著降低，但确实使LPL活性和LPL含量降低了45%。当脓毒症大鼠进食时，也观察到禁食状态下比目鱼肌中脓毒症诱导的LPL活性、LPL mRNA水平和LPL含量的抑制。结果表明，在禁食脓毒症期间，附睾脂肪以及可能比目鱼肌中的LPL表达涉及转录调控。在进食状态的脓毒症期间，脂肪组织中LPL的调节可能涉及翻译后机制。
