Pellegrino de Iraldi A, Cardoni R
Cell Tissue Res. 1979 Aug 3;200(1):91-100. doi: 10.1007/BF00236890.
Two compartments have been defined in monoaminergic synaptic vesicles: the core or central compartment, storage site for monoamines, and the matrix or outer compartment, of unknown function. The outer compartment reacts with the mixture of zinc iodide-osmium tetroxide (ZIO). This reaction is temperature and time dependent and may be abolished by -SH reagents. The effect of drugs inhibiting the synthesis of serotonin and noradrenaline (stored in the core) on the ZIO reaction in the matrix was studied in synaptic vesicles of rat pineal nerves. The inhibitors of monoamine synthesis abolish or decrease the ZIO reaction directly or in combination with the administration of tyramine. This effect is temperature dependent suggesting that the drugs act on different components of the matrix that react with ZIO at different temperatures. A comparison of the present results with those obtained with -SH reagents seems to indicate that the drugs assayed act, at least in part, by changing the accessibility of -SH groups in vesicle proteins. (An abstract of this paper was presented at the 7th International Congress of Pharmacology, Paris, 1978.)
核心或中央区室，单胺的储存部位；以及基质或外部区室，其功能未知。外部区室与碘化锌 - 四氧化锇（ZIO）混合物发生反应。该反应取决于温度和时间，并且可能被 -SH 试剂消除。在大鼠松果体神经的突触小泡中，研究了抑制血清素和去甲肾上腺素（储存于核心区室）合成的药物对基质中 ZIO 反应的影响。单胺合成抑制剂直接或与酪胺联合给药时可消除或降低 ZIO 反应。这种效应取决于温度，表明这些药物作用于基质中在不同温度下与 ZIO 反应的不同成分。将目前的结果与用 -SH 试剂获得的结果进行比较似乎表明，所检测的药物至少部分地通过改变小泡蛋白中 -SH 基团的可及性而起作用。（本文摘要曾在 1978 年于巴黎召开的第七届国际药理学大会上发表。）
