转化生长因子-β超家族成员对Hox基因表达的细胞类型特异性调控：人骨肉瘤与神经母细胞瘤细胞系的比较

Cell-type-specific modulation of Hox gene expression by members of the TGF-beta superfamily: a comparison between human osteosarcoma and neuroblastoma cell lines.

作者信息

Kloen P, Visker M H, Olijve W, van Zoelen E J, Boersma C J

机构信息

Department of Orthopaedic Surgery, Massachusetts General Hospital, and Harvard Medical School, Boston, USA.

出版信息

Biochem Biophys Res Commun. 1997 Apr 17;233(2):365-9. doi: 10.1006/bbrc.1997.6458.

DOI:10.1006/bbrc.1997.6458

PMID:9144540

Abstract

Homeobox gene expression in osteoblast-like cells was investigated using the polymerase chain reaction (PCR). A total of 13 homeobox genes was detected in U-2 OS (human osteosarcoma) and MC3T3-E1 (mouse osteoblast) cells by sequencing cloned PCR products. Using specific primers, a different pattern of Hox gene expression was shown for the neuroblastoma cell line SK-N-SH relative to U-2 OS and MC3T3-E1. Additionally, we showed that expression of HOXC6 in U-2 OS and SK-N-SH was differentially regulated by rhBMP-2, TGF-beta and activin-A. This suggests that specific Hox genes may be target genes for TGF-beta superfamily members, and allows us to further understand the complex functions of these growth factors and how they relate to growth and development.

摘要

利用聚合酶链反应（PCR）研究了成骨样细胞中的同源框基因表达。通过对克隆的PCR产物进行测序，在U-2 OS（人骨肉瘤）和MC3T3-E1（小鼠成骨细胞）细胞中总共检测到13个同源框基因。使用特异性引物，与U-2 OS和MC3T3-E1相比，神经母细胞瘤细胞系SK-N-SH显示出不同的Hox基因表达模式。此外，我们发现U-2 OS和SK-N-SH中HOXC6的表达受重组人骨形态发生蛋白-2（rhBMP-2）、转化生长因子-β（TGF-β）和激活素-A的差异调节。这表明特定的Hox基因可能是TGF-β超家族成员的靶基因，使我们能够进一步了解这些生长因子的复杂功能以及它们与生长发育的关系。