Trougakos Ioannis P, Chondrogianni Niki, Amarantos Ioannis, Blake Jonathon, Schwager Christian, Wirkner Ute, Ansorge Wilhelm, Gonos Efstathios S
Department of Cell Biology & Biophysics, Faculty of Biology, University of Athens, Panepistimiopolis Zografou, Athens 15784, Greece.
Cancer Genet Cytogenet. 2010 Jan 15;196(2):109-18. doi: 10.1016/j.cancergencyto.2009.09.012.
With the use of genome-wide cDNA microarrays, we investigated the transcriptome profile of the human osteosarcoma Sa OS and U-2 OS cell lines. In all, 1,098 chip entries were differentially regulated in the two cell lines; of these, 796 entries corresponded to characterized mRNAs. The identified genes are mostly expressed in epithelial tissues and localize on chromosomes 1, 10, and 20. Furthermore, signaling cascades for cell cycle, glycolysis, and gluconeogenesis, the p53 pathway, cell communication, and focal adhesion were found to be differently regulated in the two cell lines. The transcriptome profiles reported here provide novel information about the considerable molecular differences between these two widely used human osteosarcoma cell lines.
利用全基因组cDNA微阵列，我们研究了人骨肉瘤Sa OS和U - 2 OS细胞系的转录组图谱。总共1098个芯片条目在这两种细胞系中受到差异调节；其中796个条目对应于已鉴定的mRNA。所鉴定的基因大多在上皮组织中表达，定位于染色体1、10和20上。此外，发现细胞周期、糖酵解和糖异生、p53途径、细胞通讯和粘着斑的信号级联在这两种细胞系中受到不同调节。本文报道的转录组图谱提供了关于这两种广泛使用的人骨肉瘤细胞系之间显著分子差异的新信息。
