Shimizu K, Nomoto M, Ueta Y, Konishi T, Abe T, Gotoh S, Suzuki K, Nakamura T, Higashi K
Department of Biochemistry, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Biochem Biophys Res Commun. 1997 Apr 17;233(2):550-4. doi: 10.1006/bbrc.1997.6418.
The heat shock protein 70 (HSP70) gene expression in rat adrenal glands was investigated. In situ hybridization and Northern blotting revealed a marked increase of inducible HSP70 (HSP70-1) gene expression in the adrenal cortex, but not in the medulla, after whole body hyperthermia. In vivo footprinting assay of HSP70-1 promoter region suggested a possible involvement of other factor(s) in addition to the heat shock factor (HSF). We also detected significant increases of both ACTH and corticosterone in the plasma of the hyperthermic rats. These results suggested some physiological factors other than HSF may also participate in the tissue-specific induction of HSP70-1 gene in vivo.
对大鼠肾上腺中热休克蛋白70（HSP70）基因的表达进行了研究。原位杂交和Northern印迹分析显示，全身热疗后，肾上腺皮质中诱导型HSP70（HSP70-1）基因表达显著增加，而髓质中未增加。对HSP70-1启动子区域的体内足迹分析表明，除热休克因子（HSF）外，可能还有其他因子参与其中。我们还检测到热疗大鼠血浆中促肾上腺皮质激素（ACTH）和皮质酮均显著增加。这些结果表明，除HSF外，一些生理因素也可能参与体内HSP70-1基因的组织特异性诱导。
