Furuichi M, Nishimoto E, Koga T, Takase A, Yamashita S
Faculty of Agriculture, Institute of Biophysics, Kyushu University, Higashi-ku, Fukuoka, Japan.
Biochem Biophys Res Commun. 1997 Apr 17;233(2):555-8. doi: 10.1006/bbrc.1997.6429.
Detergent effects on the pre-crystallization of light-harvesting chlorophyll a/b-protein complex (LHCII) were investigated through the fluorescence depolarization method. Stable LHCII crystals were formed in the media containing Triton X-100 (TX) or n-nonyl-beta-D-glucopyranoside (NG) and the crystallization efficients were dependent on their concentrations. The second virial coefficient of crystallizing system, estimated by the fluorescence depolarization analysis of LHCII, showed the most harmonious value under the condition yielding the most efficient crystallization of LHCII to suggest that some specific molecular interaction leading to the crystal growth would be induced according to the concentration of TX or NG.
通过荧光去极化方法研究了去污剂对光捕获叶绿素a/b蛋白复合物（LHCII）预结晶的影响。在含有Triton X-100（TX）或正壬基-β-D-吡喃葡萄糖苷（NG）的介质中形成了稳定的LHCII晶体，且结晶效率取决于它们的浓度。通过对LHCII的荧光去极化分析估计的结晶系统的第二维里系数，在产生最有效LHCII结晶的条件下显示出最和谐的值，这表明根据TX或NG的浓度会诱导一些导致晶体生长的特定分子相互作用。
