Felipe A, Ferrer-Martínez A, Casado F J, Pastor-Anglada M
Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Spain.
Biochem Biophys Res Commun. 1997 Apr 17;233(2):572-5. doi: 10.1006/bbrc.1997.6498.
The expression of sodium-dependent purine nucleoside transport (SPNT) mRNA has been studied in physiological situations in which Na+-dependent nucleoside uptake in plasma membrane vesicles from rat liver was induced. Sodium-dependent uridine transport rates were induced in genetically obese Zucker rats, during liver regeneration after partial hepatectomy, and under euglycemic-hyperinsulinemic clamp. A PCR-generated fragment, based on a published SPNT sequence cloned from rat liver, was used as a probe in Northern blot analysis. We show that the hepatic mRNA levels of the putative sodium-dependent transport system SPNT correlate with the sodium-dependent uridine transport rates in plasma membrane vesicles from rat liver. These results suggest that the induction of the sodium-dependent nucleoside transport expressed in liver parenchymal cells involves regulation of SPNT gene expression.
在诱导大鼠肝脏质膜囊泡中钠依赖性核苷摄取的生理情况下，研究了钠依赖性嘌呤核苷转运（SPNT）mRNA的表达。在遗传性肥胖的 Zucker 大鼠中、部分肝切除术后肝脏再生期间以及正常血糖 - 高胰岛素钳夹条件下，钠依赖性尿苷转运速率均被诱导。基于从大鼠肝脏克隆的已发表的 SPNT 序列通过 PCR 生成的片段，被用作 Northern 印迹分析的探针。我们表明，假定的钠依赖性转运系统 SPNT 的肝脏 mRNA 水平与大鼠肝脏质膜囊泡中的钠依赖性尿苷转运速率相关。这些结果表明，肝实质细胞中表达的钠依赖性核苷转运的诱导涉及 SPNT 基因表达的调节。
