核酸与相关蛋白质的光交联。

Photocross-linking of nucleic acids to associated proteins.

作者信息

Meisenheimer K M, Koch T H

机构信息

Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215, USA.

出版信息

Crit Rev Biochem Mol Biol. 1997;32(2):101-40. doi: 10.3109/10409239709108550.

DOI:10.3109/10409239709108550

PMID:9145285

Abstract

Photocross-linking is a useful technique for the partial definition of the nucleic acid-protein interface of nucleoprotein complexes. It can be accomplished by one or two photon excitations of wild-type nucleoprotein complexes or by one photon excitation of nucleoprotein complexes bearing one or more substitutions with photoreactive chromophores. Chromophores that have been incorporated into nucleic acids for this purpose include aryl azides, 5-azidouracil, 8-azidoadenine, 8-azidoguanine, 4-thiouracil, 5-bromouracil, 5-iodouracil, and 5-iodocytosine. The various techniques and chromophores are described and compared, with attention to the photochemical mechanism.

摘要

光交联是一种用于部分定义核蛋白复合物核酸-蛋白质界面的有用技术。它可以通过野生型核蛋白复合物的单光子或双光子激发来实现，也可以通过带有一个或多个光反应性发色团取代的核蛋白复合物的单光子激发来实现。为此目的已掺入核酸中的发色团包括芳基叠氮化物、5-叠氮尿嘧啶、8-叠氮腺嘌呤、8-叠氮鸟嘌呤、4-硫尿嘧啶、5-溴尿嘧啶、5-碘尿嘧啶和5-碘胞嘧啶。本文描述并比较了各种技术和发色团，并关注光化学机制。