García-Robaina J C, de la Torre-Morín F, Vazquez-Moncholi C, Fierro J, Bonnet-Moreno C
Department of Allergy, Hospital Universitario Ntra. Sra. de Candelaria, Tenerife, Canary Islands, Spain.
Clin Exp Allergy. 1997 Apr;27(4):418-23.
Most published studies on changes of specific IgG or its subclasses as a response to stimuli by allergens have been performed on patients under immunotherapy. There are few reports analysing the response to immunoglobulins in patients exposed to allergens in their natural habitats.
The aim of this work was to discover the natural history of Apis specific IgG (IgG-ap) and IgG4 (IgG4-ap) levels in beekeepers from the Canary Islands.
We studied 242 beekeepers (Bks). We used a questionnaire and measured total IgE and seric Apis specific IgE (IgE-ap), IgG-ap and IgG4-ap against Apis mellifera venom.
All Bks had IgG-ap and IgG4-ap. IgE-ap was positive in 160 Bks (65.6%), but only 92 (37.6%) Bks were considered sensitized. IgG-ap and IgG4-ap showed significant correlation (r = 0.84); IgE did not correlate with IgG-ap or IgG4-ap. There was no seasonal variation in IgG-ap or IgG4-ap. The group of sensitized Bks had significantly lower IgG-ap and IgG4-ap levels (P < 0.05). The groups with longer beekeeping activity showed significantly higher levels of IgG-ap and IgG4-ap (P < 0.001). Bks with locals reactions had significantly higher IgG-ap and IgG4-ap than Bks who reported systemic reactions (P < 0.05).
Our study showed that IgG-ap and IgG4-ap appear to increase in Bks, either according to their beekeeping experience or in subjects with local reactions after bee stings.
大多数已发表的关于特异性IgG或其亚类作为变应原刺激反应变化的研究是在接受免疫治疗的患者中进行的。很少有报告分析在自然环境中接触变应原的患者对免疫球蛋白的反应。
本研究旨在发现加那利群岛养蜂人中华蜜蜂特异性IgG（IgG-ap）和IgG4（IgG4-ap）水平的自然变化规律。
我们研究了242名养蜂人。我们使用了一份问卷，并检测了总IgE以及针对意大利蜜蜂毒液的血清中华蜜蜂特异性IgE（IgE-ap）、IgG-ap和IgG4-ap。
所有养蜂人均有IgG-ap和IgG4-ap。160名养蜂人（65.6%）的IgE-ap呈阳性，但只有92名（37.6%）养蜂人被认为致敏。IgG-ap和IgG4-ap显示出显著相关性（r = 0.84）；IgE与IgG-ap或IgG4-ap无相关性。IgG-ap或IgG4-ap无季节性变化。致敏养蜂人群的IgG-ap和IgG4-ap水平显著较低（P < 0.05）。养蜂活动时间较长的人群IgG-ap和IgG4-ap水平显著较高（P < 0.001）。有局部反应的养蜂人的IgG-ap和IgG4-ap显著高于报告有全身反应的养蜂人（P < 0.05）。
我们的研究表明，IgG-ap和IgG4-ap似乎在养蜂人中会根据其养蜂经验或在被蜜蜂蜇伤后有局部反应的个体中增加。
