Pereira Santos M C, Pedro E, Spínola Santos A, Branco Ferreira M, Palma Carlos M L, Palma Carlos A G
CHIUL, Faculty of Medicine of Lisbon, Portugal.
Eur Ann Allergy Clin Immunol. 2005 Sep;37(7):273-8.
Hymenoptera venom immunotherapy (VIT) is immunologically effective in patients with systemic allergic reactions after hymenoptera stings.
To evaluate the effect of VIT on specific IgE and IgG4 immunoblotting bands in VIT-treated patients.
Specific IgE and IgG4 immunoblotting bands for hymenoptera venom were performed with ALABLOT in sera of 17 patients (8 allergic to honeybee venom, 8 to wasp and 1 to polistes venom) before and during successful VIT (1 and 3 years). Before immunotherapy, all patients had experienced moderate/severe systemic reactions to a hymenoptera sting, with positive skin tests and venom-specific IgE. During immunotherapy all patients suffered field stings, without any systemic reaction.
Before VIT we detected several immunoglobulin-binding bands in different regions, with different individual patterns. After VIT, we observed in some patients (5/8 for honeybee venom, 6/8 for wasp and 1/1 for polistes) complete disappearance of some IgE-binding bands, mainly the 15 kDa region (honeybee) and 23 and 44 kDa regions (wasp and polistes). All patients showed decreased intensity of IgE-binding bands, most pronounced in regions 16, 44 and 52 kDa (honeybee); 44 and 35 kDa bands (wasp) and 23 kDa (polistes). Some patients showed de novo appearance of IgG4-binding bands (4/8 for honeybee and 8/8 for wasp venom), mainly in 52 kDa (honeybee) and in 23 and 44 kDa regions (wasp). All patients showed increased intensity of IgG4 bands that were already present before VIT, more pronounced in 52 and 44 kDa (honeybee) and in 44 and 35 kDa regions (wasp).
During successful VIT there are changes in intensity and number of IgE and IgG4 binding bands, which could reflect the immunological improvement induced by VIT. These changes are more pronounced/frequent in wasp VIT, a fact that could explain the best results usually seen in these patients.
膜翅目昆虫毒液免疫疗法（VIT）对膜翅目昆虫叮咬后出现全身过敏反应的患者具有免疫疗效。
评估VIT对接受VIT治疗患者特异性IgE和IgG4免疫印迹条带的影响。
采用ALABLOT法检测17例患者（8例对蜜蜂毒液过敏、8例对黄蜂毒液过敏、1例对胡蜂毒液过敏）在成功进行VIT治疗前及治疗期间（1年和3年）血清中膜翅目昆虫毒液的特异性IgE和IgG4免疫印迹条带。免疫治疗前，所有患者均对膜翅目昆虫叮咬有中度/重度全身反应，皮肤试验和毒液特异性IgE呈阳性。免疫治疗期间，所有患者均遭受野外叮咬，但无任何全身反应。
VIT治疗前，我们在不同区域检测到几条免疫球蛋白结合条带，各有不同的个体模式。VIT治疗后，我们观察到部分患者（蜜蜂毒液组5/8例、黄蜂毒液组6/8例、胡蜂毒液组1/1例）的一些IgE结合条带完全消失，主要是15 kDa区域（蜜蜂）以及23和44 kDa区域（黄蜂和胡蜂）。所有患者的IgE结合条带强度均降低，在16、44和52 kDa区域（蜜蜂）最为明显；44和35 kDa条带（黄蜂）以及23 kDa条带（胡蜂）。部分患者出现了新的IgG4结合条带（蜜蜂毒液组4/8例、黄蜂毒液组8/8例），主要在52 kDa区域（蜜蜂）以及23和44 kDa区域（黄蜂）。所有患者VIT治疗前已存在的IgG4条带强度均增加，在52和44 kDa区域（蜜蜂）以及44和35 kDa区域（黄蜂）更为明显。
在成功进行VIT治疗期间，IgE和IgG4结合条带的强度和数量发生变化，这可能反映了VIT诱导的免疫改善。这些变化在黄蜂VIT治疗中更为明显/频繁，这一事实可能解释了这些患者通常取得更好疗效的原因。
