Vidruk E H, Hahn H L, Nadel J A, Sampson S R
J Appl Physiol Respir Environ Exerc Physiol. 1977 Sep;43(3):397-402. doi: 10.1152/jappl.1977.43.3.397.
The stimulant effect of histamine on intrapulmonary rapidly adapting receptors was studied in anesthetized, paralyzed, artificially ventilated dogs, to determine whether it was dependent on contraction of airway smooth muscle. Single- or few-afferent fiber recordings were made from small strands dissected from the otherwise intact vagus nerve. Aerosols of histamine increased both receptor discharge and tracheal pressure (Pt). In 27 experiments, isoproterenol prevented the increase in Pt but not in receptor activity induced by histamine. In another 32 experiments, acetylcholine (ACh) increased Pt to a greater extent than did histamine, whereas histamine increased receptor discharge more than ACh. When given as aerosol directly to the vicinity of the sensory receptor via a fiber bronchoscope, histamine stimulated 12 receptors, whereas ACh and buffer solution did not. Isoproterenol reduced slightly the stimulant effect of locally applied histamine on 8 receptors. We conclude that smooth muscle contraction is not essential for stimulant effects of histamine on rapidly adapting receptors in dog lungs, and that histamine acts directly on the receptors to increase their activity.
在麻醉、麻痹并进行人工通气的犬中研究了组胺对肺内快速适应性感受器的刺激作用，以确定其是否依赖于气道平滑肌的收缩。从完整的迷走神经中分离出的小束神经进行单根或少数传入纤维记录。组胺气雾剂可增加感受器放电和气管压力（Pt）。在27项实验中，异丙肾上腺素可阻止组胺诱导的Pt升高，但不能阻止受体活性升高。在另外32项实验中，乙酰胆碱（ACh）使Pt升高的程度大于组胺，而组胺使受体放电增加的程度大于ACh。当通过纤维支气管镜将组胺气雾剂直接给予感觉感受器附近时，组胺刺激了12个感受器，而ACh和缓冲溶液则无此作用。异丙肾上腺素略微降低了局部应用组胺对8个感受器的刺激作用。我们得出结论，平滑肌收缩对于组胺对犬肺快速适应性感受器的刺激作用并非必不可少，且组胺直接作用于感受器以增加其活性。
