Nilsson A, Trøen G, Petersen L B, Reppe S, Norum K R, Blomhoff R
Institute for Nutrition Research, University of Oslo, Norway.
Int J Biochem Cell Biol. 1997 Feb;29(2):381-9. doi: 10.1016/s1357-2725(96)00068-4.
It is suggested that cellular retinol-binding proteins are important for intracellular metabolism of retinol. Retinol bound to cellular retinol-binding proteins may be esterified with long chain fatty acids by the enzyme lecithin: retinol acyltransferase or may be oxidized to retinoic acid metabolites used in the mechanism of action of vitamin A. The aim of this present report was to determine whether altered levels of cellular retinol-binding protein type I influenced retinol storage and activation. Two different cell types have been examined after transfection with vectors producing sense or antisense mRNA for cellular retinol-binding protein type I. When HL60 cells were transfected with the expression vector for sense cellular retinol-binding protein type I high amounts of cellular retinol-binding protein type I mRNA and protein were produced. We observed that HL60 cells esterified less retinol than control cells without cellular retinol-binding protein type I. Cellular retinol-binding protein type I had, however, no effects on the proliferation or differentiation of HL60 cells by retinoids. Liver stellate cells transfected with the vector for sense cellular retinol-binding protein type I esterified more retinol than cells transfected with the expression vector for antisense cellular retinol-binding protein type I, while retinol esterification in control cells was intermediate. In conclusion, our data show that cellular retinol-binding protein type I influences retinol esterification both in liver stellate cells and in HL60 cells.
有人提出，细胞视黄醇结合蛋白对视黄醇的细胞内代谢很重要。与细胞视黄醇结合蛋白结合的视黄醇可能会被卵磷脂：视黄醇酰基转移酶用长链脂肪酸酯化，或者可能被氧化为视黄酸代谢物，用于维生素A的作用机制。本报告的目的是确定I型细胞视黄醇结合蛋白水平的改变是否会影响视黄醇的储存和激活。在用产生I型细胞视黄醇结合蛋白的正义或反义mRNA的载体转染后，对两种不同的细胞类型进行了检查。当用I型细胞视黄醇结合蛋白的正义表达载体转染HL60细胞时，产生了大量的I型细胞视黄醇结合蛋白mRNA和蛋白质。我们观察到，与没有I型细胞视黄醇结合蛋白的对照细胞相比，HL60细胞酯化的视黄醇更少。然而，I型细胞视黄醇结合蛋白对类维生素A诱导的HL60细胞增殖或分化没有影响。用I型细胞视黄醇结合蛋白的正义载体转染的肝星状细胞酯化的视黄醇比用I型细胞视黄醇结合蛋白的反义表达载体转染的细胞更多，而对照细胞中的视黄醇酯化处于中间水平。总之，我们的数据表明，I型细胞视黄醇结合蛋白在肝星状细胞和HL60细胞中均影响视黄醇酯化。
