Cunha A S, Grossiord J L, Puisieux F, Seiller M
Faculdade de farmacia da Universidade Federal de Minas Gerais, Belo Horizonte/MG, Brazil.
J Microencapsul. 1997 May-Jun;14(3):321-33. doi: 10.3109/02652049709051136.
In this work the biological effects of two w/o/w multiple emulsions composed of the soybean oil (EHS) or medium-chain triglycerides (ETCM), containing insulin, were studied. The release mechanism of insulin from multiple emulsions proposed in our previous in-vitro investigations was confirmed by subcutaneous administration. This mechanism is the swelling-breakdown phenomenon which occurs when the emulsions are diluted under hypo-osmotic condition. The biological effect after oral administration, evaluated in two experimental protocols, single administration in normal and diabetic rats and short-term treatment in diabetic rats, shows that in diabetic rats small amounts of biologically active insulin were absorbed from these emulsions. In these experiments no significant difference between EHS and ETCM was found.
在本研究中，对两种由大豆油（EHS）或中链甘油三酯（ETCM）组成、含有胰岛素的水包油包水型多重乳液的生物学效应进行了研究。我们之前体外研究所提出的胰岛素从多重乳液中的释放机制，通过皮下给药得到了证实。该机制是乳液在低渗条件下被稀释时发生的溶胀-破裂现象。在两个实验方案中评估了口服给药后的生物学效应，即正常大鼠和糖尿病大鼠单次给药以及糖尿病大鼠短期治疗，结果表明在糖尿病大鼠中，从这些乳液中吸收了少量具有生物活性的胰岛素。在这些实验中，未发现EHS和ETCM之间有显著差异。
