Sando J J, Nielsen C J, Pratt W B
J Biol Chem. 1977 Nov 10;252(21):7579-82.
The specific glucocorticoid binding capacity of the 100,000 X g supernatant from thymocytes becomes rapidly inactivated with a half-life of approximately 4 h at 0 degrees. Following this inactivation, the receptor can be reactivated by a factor (or factors) present in the high speed supernatant of L929 mouse fibroblasts. The L cell activating factor is excluded from Sephadex G-25. It can be separated from the glucocorticoid receptor of L cells by chromatography of Sephadex G-150 where it elutes with the small molecular weight material. The probable molecular weight range for the activating factor is 5,000 to 20,000. Approximately 50% of the activating activity is retained after heating at 90 degrees for 15 min.
胸腺细胞100,000×g上清液的特异性糖皮质激素结合能力在0℃下迅速失活，半衰期约为4小时。失活后，受体可被L929小鼠成纤维细胞高速上清液中存在的一种或多种因子重新激活。L细胞激活因子不能通过葡聚糖凝胶G - 25。通过葡聚糖凝胶G - 150层析可将其与L细胞的糖皮质激素受体分离，它与小分子物质一起洗脱。激活因子的可能分子量范围为5000至20000。在90℃加热15分钟后，约50%的激活活性得以保留。
