采用薄层色谱、气液色谱和质谱法分析C19O3类固醇。

Analysis of C19O3 steroids by thin-layer and gas-liquid chromatography and mass spectrometry.

作者信息

Kerebel A, Morfin R F, Berthou F L, Picart D, Bardou L G, Floch H H

出版信息

J Chromatogr. 1977 Oct 21;140(3):229-44. doi: 10.1016/s0021-9673(00)93582-9.

Abstract

The separation of twenty-six saturated and two unsaturated C19O3 steroids has been studied by thin-layer chromatography on silica gel F254, with seven mobile phases, and by gas-liquid chromatography on packed columns with four stationary phases; combination of both techniques permitted separation of all the test compounds. The mass spectra of the steroids were obtained by gas chromatography-mass spectrometry and are presented. Fragmentation processes have been studied, and characteristic ions that may be used for multiple ion detection or lead to identification of biologically produced C19O3 steroids are discussed.

摘要

通过在硅胶F254上采用七种流动相进行薄层色谱法，以及在填充柱上采用四种固定相进行气液色谱法，对26种饱和和2种不饱和C19O3甾体进行了分离研究；两种技术的结合使得所有测试化合物都能得到分离。通过气相色谱 - 质谱联用获得了甾体的质谱图并予以呈现。对裂解过程进行了研究，并讨论了可用于多离子检测或有助于鉴定生物产生的C19O3甾体的特征离子。