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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Chelsea Department of Pharmacy, King's College London, UK.

Xenobiotica. 1997 Apr;27(4):389-99. doi: 10.1080/004982597240541.

During the in vitro microsomal metabolism of the secondary alicyclic amines, nornicotine, anabasine or 4-benzylpiperidine in the presence of cyanide, a new product was formed. 2. Comparison of these new products with authentic compounds by hplc and ms showed they were the corresponding 1'-N-cyanomethyl compounds. 3. The source of the methylene group was shown to be formaldehyde, arising during incubation, from either microsomal lipid and/or glycerol used to store microsomes for long periods at low temperature. 4. The formation of the 1'-N-cyanomethyl compounds was enhanced in the presence of substrates producing formaldehyde during their metabolism, in direct relationship to the amount of formaldehyde generated. 5. The results indicate that care should be taken in interpreting data obtained from incubates containing secondary amines and cyanide, especially in the presence of substrates capable of donating formaldehyde.

在含有氰化物的情况下，对仲脂环胺、降烟碱、新烟草碱或4-苄基哌啶进行体外微粒体代谢时，会形成一种新产物。2. 通过高效液相色谱法（HPLC）和质谱法（MS）将这些新产物与标准化合物进行比较，结果表明它们是相应的1'-N-氰甲基化合物。3. 亚甲基的来源被证明是甲醛，甲醛是在孵育过程中，由微粒体脂质和/或用于在低温下长期储存微粒体的甘油产生的。4. 在代谢过程中产生甲醛的底物存在时，1'-N-氰甲基化合物的形成会增强，且与产生的甲醛量直接相关。5. 结果表明，在解释从含有仲胺和氰化物的孵育物中获得的数据时应谨慎，特别是在存在能够提供甲醛的底物的情况下。

Chelsea Department of Pharmacy, King's College London, UK.

Xenobiotica. 1997 Apr;27(4):389-99. doi: 10.1080/004982597240541.

During the in vitro microsomal metabolism of the secondary alicyclic amines, nornicotine, anabasine or 4-benzylpiperidine in the presence of cyanide, a new product was formed. 2. Comparison of these new products with authentic compounds by hplc and ms showed they were the corresponding 1'-N-cyanomethyl compounds. 3. The source of the methylene group was shown to be formaldehyde, arising during incubation, from either microsomal lipid and/or glycerol used to store microsomes for long periods at low temperature. 4. The formation of the 1'-N-cyanomethyl compounds was enhanced in the presence of substrates producing formaldehyde during their metabolism, in direct relationship to the amount of formaldehyde generated. 5. The results indicate that care should be taken in interpreting data obtained from incubates containing secondary amines and cyanide, especially in the presence of substrates capable of donating formaldehyde.

在含有氰化物的情况下，对仲脂环胺、降烟碱、新烟草碱或4-苄基哌啶进行体外微粒体代谢时，会形成一种新产物。2. 通过高效液相色谱法（HPLC）和质谱法（MS）将这些新产物与标准化合物进行比较，结果表明它们是相应的1'-N-氰甲基化合物。3. 亚甲基的来源被证明是甲醛，甲醛是在孵育过程中，由微粒体脂质和/或用于在低温下长期储存微粒体的甘油产生的。4. 在代谢过程中产生甲醛的底物存在时，1'-N-氰甲基化合物的形成会增强，且与产生的甲醛量直接相关。5. 结果表明，在解释从含有仲胺和氰化物的孵育物中获得的数据时应谨慎，特别是在存在能够提供甲醛的底物的情况下。