Kraak J C, Jonker K M, Huber J K
J Chromatogr. 1977 Nov 11;142:671-88. doi: 10.1016/s0021-9673(01)92076-x.
The retention behaviour of amino acids in phase systems consisting of a hydrophobic solid support as the stationary phase and water-organic solvent mixtures containing a small amount of an anionic detergent as the mobile phase was investigated. Such phase systems are found to behave like conventional ion-exchange systems. The degree and order of retention of amino acids can be influenced by changing the temperature, the nature of the hydrophobic support, the pH and the nature and concentration of the anionic detergent, organic constituent and counter ion in the eluent. In many instances this solvent-generated (dynamic) ion-exchange chromatography shows a greater selectivity than conventional ion-exchange systems towards amino acids. The results obtained so far indicate that a complete separation of the 19 protein amino acids by applying solvent gradients or/and multi-column system is possible within 30 min.
研究了氨基酸在以疏水固体载体为固定相、含有少量阴离子洗涤剂的水-有机溶剂混合物为流动相的相体系中的保留行为。发现此类相体系的行为类似于传统的离子交换体系。改变温度、疏水载体的性质、pH值以及洗脱液中阴离子洗涤剂的性质和浓度、有机成分及抗衡离子，可影响氨基酸的保留程度和顺序。在许多情况下，这种溶剂产生的（动态）离子交换色谱法对氨基酸的选择性比传统离子交换体系更高。目前所获得的结果表明，通过应用溶剂梯度或/和多柱系统，有可能在30分钟内将19种蛋白质氨基酸完全分离。
