Doury-Berthod M, Poitrenaud C, Tremillon B
J Chromatogr. 1979 Nov 7;179(1):37-61. doi: 10.1016/s0021-9673(00)80656-1.
The ligand-exchange separation of amino acids in protein hydrolysates has been studied in ammoniacal media. The various factors influencing the chromatographic behaviour have been investigated. The selectivity of the process and the column efficiency depend appreciably on the resin matrix and the nature of the functional groups. The best results have been obtained with the acrylic-type Bio Rex 70 resin, in the copper(II) form. The zinc(II) form of this support has also been tested; the elution order of glycine, alanine and leucine is the same as with the copper(II) form, but the affinities of these molecules for the zinc(II) resin are weaker. The influence of the concentrations of copper(II) and ammonia on the elution volumes obtained for all the amino acids with the copper(II) Bio Rex 70 resin has been studied. The general variation of retention and selectivity with eluent composition confirms the theoretical expressions established previously. Ligand-exchange chromatography has been compared to the conventional ion-exchange process using acidic or basic species. This study reveals analogies and differences between both techniques, and shows that ligand exchange can provide new opportunities for the separation of amino acids.
已在氨性介质中研究了蛋白质水解产物中氨基酸的配体交换分离。研究了影响色谱行为的各种因素。该过程的选择性和柱效率在很大程度上取决于树脂基质和官能团的性质。使用丙烯酸型Bio Rex 70树脂（铜（II）形式）可获得最佳结果。也测试了该载体的锌（II）形式；甘氨酸、丙氨酸和亮氨酸的洗脱顺序与铜（II）形式相同，但这些分子对锌（II）树脂的亲和力较弱。研究了铜（II）和氨的浓度对使用铜（II）Bio Rex 70树脂获得的所有氨基酸洗脱体积的影响。保留率和选择性随洗脱液组成的总体变化证实了先前建立的理论表达式。已将配体交换色谱法与使用酸性或碱性物质的传统离子交换过程进行了比较。这项研究揭示了两种技术之间的异同，并表明配体交换可为氨基酸的分离提供新的机会。
