Strohner P, Sarrach D, Reich J G
BioTez Berlin-Buch GmbH, Germany.
J Immunol Methods. 1997 Apr 25;203(2):113-22. doi: 10.1016/s0022-1759(97)00022-7.
Affinity parameters obtained from standard calibration curves of radioimmunoassays (RIA) have been shown to decrease with increasing antibody concentration. A modified binding model was introduced in which not only binding capacity but also affinity was influenced by the antibody concentration. This allows a consistent overall description of RIA standards and titration curves for the characterization of binding assays.
从放射免疫分析(RIA)标准校准曲线获得的亲和参数已显示会随着抗体浓度的增加而降低。引入了一种改进的结合模型,其中不仅结合能力而且亲和力都受抗体浓度影响。这使得能够对用于结合分析表征的RIA标准品和滴定曲线进行连贯的整体描述。