Ishihama A
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
Biochimie. 1996;78(11-12):1097-102. doi: 10.1016/s0300-9084(97)86735-1.
Influenza virus-associated RNA polymerase is composed of one molecule each of three viral P proteins and carries the complete activity of capped RNA-primed vRNA-directed transcription. The RNA polymerase holoenzyme also carries capped RNA endonuclease to generate capped oligonucleotide primers for transcription and 3'-to-5' exonuclease to remove erroneously polymerized nucleotides at nascent RNA 3' termini prior to the addition of correct substrates. PB1 is the core subunit for not only RNA synthesis but also the assembly of PB2 and PA into the holoenzyme complex, while PB2 plays a key role in capped RNA cleavage. The transcriptase is converted into the RNA replicase with the full activity of replication, ie vRNA-directed cRNA synthesis and cRNA-directed vRNA synthesis, after interaction with an as yet unidentified host factor(s).
流感病毒相关的RNA聚合酶由三种病毒P蛋白各一个分子组成，并具有带帽RNA引发的vRNA指导转录的完整活性。RNA聚合酶全酶还携带带帽RNA内切核酸酶以生成用于转录的带帽寡核苷酸引物，以及3'至5'外切核酸酶以在添加正确底物之前去除新生RNA 3'末端错误聚合的核苷酸。PB1不仅是RNA合成的核心亚基，也是PB2和PA组装成全酶复合物的核心亚基，而PB2在带帽RNA切割中起关键作用。在与尚未鉴定的宿主因子相互作用后，转录酶转变为具有完整复制活性的RNA复制酶，即vRNA指导的cRNA合成和cRNA指导的vRNA合成。
