Chiang B L, Ding H J, Chou C C, Hsieh K H
Department of Pediatrics, National Taiwan University College of Medicine, Taipei, R.O.C.
Pediatr Allergy Immunol. 1996 Nov;7(4):193-8. doi: 10.1111/j.1399-3038.1996.tb00132.x.
Allergen-specific T cells have been thought to play a central role in the pathogenesis of asthma. It has been well documented that allergen-specific T cells derived from atopic patients are predominantly of type 2 T helper cell pattern. However, allergen-specific T cells derived in nonatopic normals are of type 1 in contrast to atopic patients. The purpose of the study was to develop and characterize both mite allergen-specific TH1 and TH2 clones from the same asthmatic child. With exogenous supplemental cytokine, both TH1 and TH2 clones from the same patient have been developed and maintained in this laboratory for more than one year. All these T cell clones showed dose dependent allergen-specific proliferative response and expressed with CD4+, CD45RA- markers. Elucidation of the origin and interaction between these two different types of T helper cells might shed light on understanding the pathogenesis of atopic diseases and the mechanisms of hyposensitization in atopic patients.
变应原特异性T细胞被认为在哮喘发病机制中起核心作用。有充分文献记载，来自特应性患者的变应原特异性T细胞主要呈2型辅助性T细胞模式。然而，与特应性患者相比，非特应性正常人产生的变应原特异性T细胞呈1型。本研究的目的是从同一哮喘儿童中培养并鉴定螨变应原特异性TH1和TH2克隆。通过外源性补充细胞因子，本实验室已从同一患者培养并维持TH1和TH2克隆一年多。所有这些T细胞克隆均表现出剂量依赖性变应原特异性增殖反应，并表达CD4 +、CD45RA -标志物。阐明这两种不同类型辅助性T细胞的起源及相互作用可能有助于理解特应性疾病的发病机制以及特应性患者脱敏的机制。
