Kampinga J, Groen H, Klatter F A, Pater J M, van Petersen A S, Roser B, Nieuwenhuis P, Aspinall R
Department of Histology and Cell Biology, University of Groningen, The Netherlands.
Thymus. 1997;24(3):173-200.
The presence or absence of CD4, CD8, Thy-1, RT6 and CD45RC revealed a number of T-cell subpopulations in the rat. Vascular thymus transplantation was used in RT7 congenics to establish the lineage relationship between these subpopulations by following phenotypic changes after thymus emigration. We found that recent thymic emigrants exhibit the Thy-1+/RT6-/CD45RC- phenotype and express either CD4 or CD8. Within 11 days after emigration, these cells differentiated into Thy-1-/RT6+/CD45RC+ cells. From 33 to 76 days following transplantation, a proportion of the latter lost RT6 and/or CD45RC expression, suggesting further differentiation. The pathway of 'mature' T-cell differentiation could be reconstructed from these data and analysis of the differences between T-cell subsets in thymectomized and normal control rats. End-stages of post-thymic T-cell differentiation in the rat were most likely to be Thy-1-/RT6+/CD45RC- and Thy-1-/RT6-/CD45RC+ T cells.
CD4、CD8、Thy-1、RT6和CD45RC的有无揭示了大鼠体内的一些T细胞亚群。在RT7同源系中采用血管胸腺移植，通过追踪胸腺移出后的表型变化来确定这些亚群之间的谱系关系。我们发现，近期胸腺移出细胞呈现Thy-1+/RT6-/CD45RC-表型，并表达CD4或CD8。移出后11天内，这些细胞分化为Thy-1-/RT6+/CD45RC+细胞。移植后33至76天，一部分后者失去RT6和/或CD45RC表达，提示进一步分化。根据这些数据以及对胸腺切除大鼠和正常对照大鼠T细胞亚群差异的分析，可以重建“成熟”T细胞的分化途径。大鼠胸腺后T细胞分化的终末阶段最有可能是Thy-1-/RT6+/CD45RC-和Thy-1-/RT6-/CD45RC+ T细胞。
