Nakada Y, Tsukatani Y, Kosaka T, Kuwabara M, Tanaka S, Fujiwara K
Department of Veterinary Radiology, College of Agriculture, Nihon University, Kanagawa, Japan.
Vet Immunol Immunopathol. 1997 Mar;55(4):273-82. doi: 10.1016/s0165-2427(96)05720-0.
The relationship between radical production and natural killer cytotoxic factor (NKCF) release via canine natural killer (NK)-mediated cytotoxic mechanism was examined. Radical production and NKCF release was induced in NK cells stimulated with either dead target cells, or their cytoplasmic membranes, as well as live target cells. Canine NKCF evoked target cell lysis but did not induce radical production. Radical production was inhibited by the addition of Tiron or n-propyl gallate, whereas NK-mediated cytotoxicity and NKCF release were only inhibited by the addition of n-propyl gallate. These results suggested that radical production and NKCF release may be induced by the contact and binding of NK cells to the target cell cytoplasmic membrane. Therefore, the release of NKCF from NK cells attached to the target cell cytoplasmic membrane may be associated with the production of radicals, especially hydroxyl radicals.
研究了通过犬自然杀伤（NK）介导的细胞毒性机制产生自由基与释放自然杀伤细胞细胞毒性因子（NKCF）之间的关系。在用死靶细胞、其细胞质膜或活靶细胞刺激的NK细胞中诱导自由基产生和NKCF释放。犬NKCF引起靶细胞裂解，但不诱导自由基产生。加入钛铁试剂或没食子酸正丙酯可抑制自由基产生，而加入没食子酸正丙酯仅抑制NK介导的细胞毒性和NKCF释放。这些结果表明，自由基产生和NKCF释放可能是由NK细胞与靶细胞质膜的接触和结合诱导的。因此，附着于靶细胞质膜的NK细胞释放NKCF可能与自由基尤其是羟基自由基的产生有关。
