Higa O Z, Kumakura M
Comissao Nacional De Energia Nuclear, CNEN/SP, Cidade Universitaria, Armando De Salles Oliveira, Sao Paulo, Brazil.
Biomaterials. 1997 May;18(9):697-700. doi: 10.1016/s0142-9612(96)00184-6.
A preparation method of immobilized urease discs by an electron beam irradiation technique was developed, and the relationship between enzyme activity and preparation conditions was investigated. The immobilized urease disc was a thin circular film (200 microm, 5 mm phi) that is useful for biomedical applications. The activity of urease irradiated with 1 Mrad at room temperature was protected by the presence of cysteine. The activity of the immobilized urease discs was studied as a function of monomer concentration (80-90%) and the thicker disc gave a high activity. The durability of the immobilized urease discs gave a high activity. The durability of the immobilized urease discs was evaluated by repeated batch enzyme reactions, and a high activity yield (80-85%) was obtained.
开发了一种通过电子束辐照技术制备固定化脲酶圆盘的方法，并研究了酶活性与制备条件之间的关系。固定化脲酶圆盘是一种薄圆形薄膜（200微米，直径5毫米），可用于生物医学应用。在室温下用1兆拉德辐照的脲酶活性受到半胱氨酸的保护。研究了固定化脲酶圆盘的活性与单体浓度（80 - 90%）的关系，较厚的圆盘具有较高的活性。固定化脲酶圆盘的耐久性通过重复批次酶反应进行评估，获得了较高的活性产率（80 - 85%）。
