Henson P M, Pinckard R N
J Immunol. 1977 Dec;119(6):2179-84.
The anaphylactic response to an i.v. injection of antigen into rabbits making only IgE antibody against the antigen was shown to be preceded by a disappearance of the metachromatic staining properties of the circulating basophils and was accompanied by marked but transient thrombocytopenia. The platelets which returned to the circulation 1 hr after the anaphylaxis were shown to be unresponsive to the secretion-inducing activity of basophil-derived platelet-activating factor (PAF) when compared with platelets examined before antigen challenge. By contrast, platelet responsiveness to other stimuli such as collagen, thrombin, and C3b was unchanged. The specific desensitization to PAF provides strong evidence for the action of this mediator on platelets in vivo during IgE-mediated anaphylaxis and provides a useful tool for detecting the effects of particular cell activators in inflammatory reactions.
将抗原静脉注射到仅产生针对该抗原的IgE抗体的兔体内，其过敏反应表现为循环嗜碱性粒细胞异染性染色特性消失，同时伴有明显但短暂的血小板减少。与抗原激发前检查的血小板相比，过敏反应1小时后回到循环中的血小板对嗜碱性粒细胞衍生的血小板活化因子（PAF）的分泌诱导活性无反应。相比之下，血小板对其他刺激物如胶原蛋白、凝血酶和C3b的反应性未改变。对PAF的特异性脱敏为该介质在IgE介导的过敏反应期间体内对血小板的作用提供了有力证据，并为检测炎症反应中特定细胞激活剂的作用提供了有用的工具。
