Kawakura K, Miyake Y, Murakami R K, Kondoh S, Hirata T I, Kaneda Y
National Institute of Animal Industry, Tsukuba, Japan.
Cytogenet Cell Genet. 1997;76(1-2):36-8. doi: 10.1159/000134508.
The structure of the Y chromosome was investigated by FISH and G banding in 3 cases of bovine XY females shown by PCR to lack the SRY gene. Although 2 different repeat-DNA sequences (BC1.2 and btDYZ-1) hybridized to the short arm of the Y chromosome in a normal bull, they hybridized to both arms of the Y chromosome in the XY females. In the Y chromosome from XY females, only the centromeric region was darkly stained by G banding, in comparison with dark staining on the long arm of a normal Y chromosome. From the results obtained, it seemed that the Y chromosome from the XY females was structurally abnormal, namely an isochromosome with an identical short arm. Our results also confirm that SRY is located on the long arm of the Y chromosome in bovine.
通过荧光原位杂交（FISH）和G带分析，对3例经聚合酶链反应（PCR）检测显示缺乏SRY基因的牛XY雌性个体的Y染色体结构进行了研究。虽然在正常公牛中，2种不同的重复DNA序列（BC1.2和btDYZ - 1）与Y染色体的短臂杂交，但在XY雌性个体中，它们与Y染色体的两条臂都杂交。与正常Y染色体长臂上的深染相比，XY雌性个体的Y染色体仅着丝粒区域被G带深染。根据所得结果，XY雌性个体的Y染色体似乎在结构上异常，即具有相同短臂的等臂染色体。我们的结果还证实，SRY基因位于牛Y染色体的长臂上。
