Fladung M, Ahuja M R
Federal Research Centre for Forestry and Forest Products, Institute of Forest Genetics, Grosshansdorf, Germany.
Plant Mol Biol. 1997 Apr;33(6):1097-103. doi: 10.1023/a:1005788706864.
The transposable element Ac from maize, in combination with the phenotypic selectable marker rolC, was employed in transformation experiments of a hybrid aspen clone. A number of transgenic clones exhibited light-green sectors on green leaves. In vitro regeneration from leaves showing a high number of light-green spots resulted in R2 plants, which also showed light-green sectored leaves. However, only one out of 385 regenerated plants obtained showed green leaves. Both PCR and northern analysis indicated Ac excision and restoration of rolC expression. In Southern blot analysis of this green plant additional bands were observed as compared to the original R1 plant. The occurrence of these bands and a suggested Ac excision in the non-green L1-epidermal layer leading to periclinal chimerism of this plant is discussed.
来自玉米的转座元件Ac与表型选择标记rolC相结合，用于杂交白杨无性系的转化实验。许多转基因无性系在绿色叶片上出现浅绿色扇形区域。从显示大量浅绿色斑点的叶片进行离体再生得到了R2植株，这些植株也表现出浅绿色扇形叶。然而，在获得的385株再生植株中只有1株表现出绿色叶片。PCR和Northern分析均表明Ac发生了切除且rolC表达得以恢复。与原始R1植株相比，在对这株绿色植株进行的Southern印迹分析中观察到了额外的条带。本文讨论了这些条带的出现以及在非绿色L1表皮层中推测的Ac切除导致该植株周缘嵌合体的情况。
