Okon E B, Marjanovic V, Hranisavljevic J, Vucelic D
Biokhimiia. 1996 Dec;61(12):2082-91.
Silver staining of proteins separated by electrophoresis in the thin layer of polyacrylamide gel can be quantitatively measured when the results are statistically treated. In the mathematical model, the reduction of protein-bound silver ions (crucial silver staining step) is considered to be autocatalytic. According to the model, the reaction rate strongly depends on the stability constant of silver complexes with functional groups of protein amino acids; and the shape of the calibration curve is determined by the ratio of concentration of silver-binding side chain groups to the reciprocal value of the stability constant. The complex shape of the curves can be explained the combination of autocatalysis and saturation. High sensitivity of this reaction to experimental conditions, contribution of complexes with different stability constants to the intensity of protein band staining, and optical effects are analyzed.
在聚丙烯酰胺凝胶薄层中通过电泳分离的蛋白质进行银染色时，若对结果进行统计学处理，便可进行定量测定。在该数学模型中，蛋白质结合银离子的还原（关键的银染色步骤）被认为是自催化的。根据该模型，反应速率强烈依赖于银与蛋白质氨基酸官能团形成的络合物的稳定常数；校准曲线的形状由银结合侧链基团的浓度与稳定常数倒数的比值决定。曲线的复杂形状可通过自催化和饱和作用的结合来解释。分析了该反应对实验条件的高敏感性、不同稳定常数的络合物对蛋白质条带染色强度的贡献以及光学效应。
