以C18硅胶为基质的金鸡纳生物碱光传感器。

Optosensor for cinchona alkaloids with C18 silica gel as a substrate.

作者信息

Gong Z, Zhang Z, Yang X

机构信息

Department of Chemistry, Nanjing University, China.

出版信息

Analyst. 1997 Mar;122(3):283-5. doi: 10.1039/a604450f.

DOI:10.1039/a604450f

PMID:9158243

Abstract

A flow-through optosensor for cinchona alkaloids with C18 silica gel as a substrate is proposed. The sensor is developed in conjunction with a flow-injection analysis system and is based on the retention of the cinchona alkaloids on a C18 column and the enhancement of their fluorescence. The analytical performance characteristics of the proposed sensor for the detection and quantification of these alkaloids were as follows: the detection limits of quinine, cinchonine, quinidine and cinchonidine were 2.3, 31.6, 2.3 and 31.6 ng ml-1, respectively, with relative standard deviations of 0.9% for quinine and quinidine (20 ng ml-1, n = 7) and 1.1% for cinchonine and cinchonidine (4.0 micrograms ml-1, n =7), respectively. Most of the common species did not interfere. The recommended method has been successfully tested for determination of quinine in pharmaceutical preparations and soft drinks.

摘要

提出了一种以C18硅胶为基质的用于金鸡纳生物碱的流通式光传感器。该传感器与流动注射分析系统联合开发，基于金鸡纳生物碱在C18柱上的保留及其荧光增强。所提出的用于检测和定量这些生物碱的传感器的分析性能特征如下：奎宁、辛可宁、奎尼丁和辛可尼丁的检测限分别为2.3、31.6、2.3和31.6 ng ml-1，奎宁和奎尼丁（20 ng ml-1，n = 7）的相对标准偏差分别为0.9%，辛可宁和辛可尼丁（4.0 μg ml-1，n = 7）的相对标准偏差分别为1.1%。大多数常见物质不产生干扰。所推荐的方法已成功用于药物制剂和软饮料中奎宁的测定。