Knoll W, Stark G
J Membr Biol. 1977 Oct 3;37(1):13-28. doi: 10.1007/BF01940921.
Temperature jump relaxation experiments on planar lipid membranes in the presence of valinomycin were performed using the absorption of a strong light flash as an energy source for the generation of the T-jump. The relaxation of the current carried by valinomycin/Rb+ complexes was measured. The results were interpreted on the basis of a transport model which was also analyzed by voltage jump relaxation experiments. The study shows that the application of the T-jump technique provides valuable information about transport kinetics as well as the dynamics of the membrane structure. At the given experimental conditions the relaxation of the current is believed to reflect a temperature-dependent transition of the membrane to a new conformational state of low order. The relaxation could be resolved with the present technique only at low temperatures and for membranes of high microviscosity.
在缬氨霉素存在的情况下，利用强光脉冲的吸收作为产生温度跃变（T-jump）的能量源，对平面脂质膜进行了温度跃变弛豫实验。测量了缬氨霉素/Rb⁺复合物携带的电流弛豫。结果基于一个传输模型进行了解释，该模型也通过电压跃变弛豫实验进行了分析。研究表明，温度跃变技术的应用提供了有关传输动力学以及膜结构动力学的有价值信息。在给定的实验条件下，电流弛豫被认为反映了膜向低序新构象状态的温度依赖性转变。只有在低温和高微粘度的膜中，才能用本技术解析弛豫过程。
