基于5S rRNA基因序列，利用异源双链分析对军团菌进行分类。
Use of heteroduplex analysis to classify legionellae on the basis of 5S rRNA gene sequences.
作者信息
Pinar A, Ahkee S, Miller R D, Ramirez J A, Summersgill J T
机构信息
Department of Medicine, University of Louisville School of Medicine, Kentucky 40292, USA.
出版信息
J Clin Microbiol. 1997 Jun;35(6):1609-11. doi: 10.1128/jcm.35.6.1609-1611.1997.
Abstract
Seventeen different species of Legionella, 12 serogroups of Legionella pneumophila, and 2 Legionella-like amoebal pathogens (LLAP1 and Sarcobium lyticum) were examined by heteroduplex analysis of PCR products of the 5S rRNA gene. Eight different banding patterns were identified, indicating that heteroduplex analysis of this gene can be used to classify these bacteria according to base substitutions between species. This classification may have future applications in clinical and epidemiological studies.
摘要
通过对5S rRNA基因的PCR产物进行异源双链分析，检测了17种不同的军团菌属物种、12个嗜肺军团菌血清群以及2种军团菌样阿米巴病原体（LLAP1和溶胞肉杆菌）。鉴定出8种不同的条带模式，表明该基因的异源双链分析可用于根据物种间的碱基替换对这些细菌进行分类。这种分类可能在临床和流行病学研究中有未来的应用。
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本文引用的文献
1
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Diagn Microbiol Infect Dis. 1996 Jan;24(1):7-14. doi: 10.1016/0732-8893(95)00254-5.
2
Legionella-like amebal pathogens--phylogenetic status and possible role in respiratory disease.军团菌样阿米巴病原体——系统发育地位及在呼吸道疾病中的可能作用。
Emerg Infect Dis. 1996 Jul-Sep;2(3):225-30. doi: 10.3201/eid0203.960311.
3
Evaluation of commercial amplification kit for detection of Legionella pneumophila in clinical specimens.用于检测临床标本中嗜肺军团菌的商用扩增试剂盒的评估
J Clin Microbiol. 1994 Jun;32(6):1503-5. doi: 10.1128/jcm.32.6.1503-1505.1994.
4
Characterization of rifampin-resistance in pathogenic mycobacteria.致病性分枝杆菌中利福平耐药性的特征分析。
Antimicrob Agents Chemother. 1994 Oct;38(10):2380-6. doi: 10.1128/AAC.38.10.2380.
5
The nucleotide sequence of the 5S rRNA from Legionella pneumophila.嗜肺军团菌5S核糖体RNA的核苷酸序列。
Nucleic Acids Res. 1987 Feb 11;15(3):1335. doi: 10.1093/nar/15.3.1335.
6
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7
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8
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J Clin Microbiol. 1992 Dec;30(12):3280-3. doi: 10.1128/jcm.30.12.3280-3283.1992.
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