Rowlett J K, Mattingly B A, Bardo M T
Department of Psychiatry and Human Behavior, University of Mississippi Medical Center, Jackson 39216, USA.
Pharmacol Biochem Behav. 1997 May-Jun;57(1-2):13-8. doi: 10.1016/s0091-3057(96)00397-8.
In two experiments, the effects of repeated apomorphine treatments on locomotor activity and terminal field dopamine synthesis was assessed after either a 1- or 15-day withdrawal period. In the first experiment, rats (n = 11/group) were treated with apomorphine (1.0 mg/kg, s.e.) or vehicle and tested for locomotor activity daily for 10 days. Fifteen days after the last repeated treatment, all rats received 1.0 mg/kg apomorphine and were tested for locomotor activity. Locomotor sensitization developed over the 10 day period and was still evident after the 15-day withdrawal period. In the second experiment rats (n = 11/group) were treated with apomorphine (1.0 mg/kg, s.c.) or vehicle and tested for locomotor activity daily for 10 days. Dopamine synthesis was assessed following 1 or 15 days of withdrawal by measuring dihydroxyphenylalanine (DOPA) accumulation (after DOPA decarboxylase inhibition with NSD-1015) in striatum and nucleus accumbens-olfactory tuberele. As in the first experiment, rats treated with repeated apomorphine showed locomotor sensitization over the 10 days, relative to controls. Dopamine synthesis was reliably enhanced in the striatum, but not nucleus accumbens-olfactory tuberele, following both 1- and 15-day withdrawal periods. These results indicate that enhanced basal dopamine synthesis following repeated apomorphine treatments, similar to locomotor sensitization, is a persistent phenomenon.
在两项实验中，评估了重复给予阿扑吗啡治疗后，经过1天或15天戒断期对运动活性和终末区域多巴胺合成的影响。在第一个实验中，大鼠（每组n = 11）接受阿扑吗啡（1.0毫克/千克，皮下注射）或赋形剂治疗，并连续10天每天测试其运动活性。在最后一次重复治疗15天后，所有大鼠接受1.0毫克/千克阿扑吗啡，并测试其运动活性。运动敏化在10天期间逐渐形成，并且在15天戒断期后仍然明显。在第二个实验中，大鼠（每组n = 11）接受阿扑吗啡（1.0毫克/千克，皮下注射）或赋形剂治疗，并连续10天每天测试其运动活性。在戒断1天或15天后，通过测量纹状体和伏隔核 - 嗅结节中（在用NSD - 1015抑制多巴脱羧酶后）二羟基苯丙氨酸（DOPA）的积累来评估多巴胺合成。与第一个实验一样，相对于对照组，重复给予阿扑吗啡治疗的大鼠在10天内表现出运动敏化。在1天和15天戒断期后，纹状体中的多巴胺合成均可靠地增强，但伏隔核 - 嗅结节中未增强。这些结果表明，重复给予阿扑吗啡治疗后基础多巴胺合成增强，类似于运动敏化，是一种持续的现象。
