Brochu M, Lehoux J G, Picard S
Research Center, Sainte-Justine's Hospital, University of Montreal, Quebec, Canada.
Endocrinology. 1997 Jun;138(6):2354-8. doi: 10.1210/endo.138.6.5198.
In the present study, the effects of gestation on various enzymes implicated in corticosteroid synthesis were evaluated in adrenal zona glomerulosa and zona fasciculata-reticularis of the Sprague-Dawley rat. The activity and expression of cholesterol side-chain cleavage cytochrome P450, 11beta-hydroxylase cytochrome P450, and aldosterone synthase cytochrome P450 (P450aldo) were analyzed. Plasma aldosterone levels were increased significantly at 22 days gestation (n = 10) and fell below the nonpregnant levels at 18-36 h postpartum (n = 11). The activity and expression of 11beta-hydroxylase cytochrome P450 and cholesterol side-chain cleavage cytochrome P450 were not modified by gestation. P450aldo activity increased at 14 days gestation (n = 4) and returned to the prepregnancy level at 2 weeks postpartum (n = 5). As shown by Northern blot analysis (n = 3), P450aldo messenger RNA increased significantly at 22 days gestation and decreased 18-36 h postpartum. We clearly demonstrated that elevated plasma aldosterone levels during pregnancy are associated with augmented activity and messenger RNA levels of P450aldo in the zona glomerulosa.
在本研究中，我们评估了妊娠对斯普拉格-道利大鼠肾上腺球状带和束状带-网状带中参与皮质类固醇合成的各种酶的影响。分析了胆固醇侧链裂解细胞色素P450、11β-羟化酶细胞色素P450和醛固酮合酶细胞色素P450（P450aldo）的活性和表达。妊娠22天时血浆醛固酮水平显著升高（n = 10），产后18 - 36小时降至非妊娠水平以下（n = 11）。妊娠对11β-羟化酶细胞色素P450和胆固醇侧链裂解细胞色素P450的活性和表达没有影响。妊娠14天时P450aldo活性增加（n = 4），产后2周恢复到妊娠前水平（n = 5）。如Northern印迹分析所示（n = 3），妊娠22天时P450aldo信使核糖核酸显著增加，产后18 - 36小时降低。我们清楚地证明，孕期血浆醛固酮水平升高与球状带中P450aldo的活性增强和信使核糖核酸水平升高有关。
