Christiansen T, Stødkilde-Jørgensen H, Flyvbjerg A
Experimental MRI-Center, University Hospital, Skejby Sygehus, Denmark.
J Endocrinol. 1997 May;153(2):193-8. doi: 10.1677/joe.0.1530193.
The aim of the present study was to use magnetic resonance imaging as a non-invasive measure of renal enlargement and regression in experimental diabetes in rats. Rats were randomized into three groups: (1) non-diabetic control animals, (2) diabetic animals, untreated for 30 days after streptozotocin treatment and (3) diabetic animals treated with insulin from day 21 to day 30 after streptozotocin administration. Magnetic resonance images were obtained from anaesthetized rats and kidney, cortex, medulla and pelvis volumes were calculated throughout the experiment until day 30. In untreated diabetic animals, a marked increase in kidney volume was seen 4 days after the induction of diabetes (P < 0.05) and after 30 days this increase amounted to 73%, with similar increases in the cortex (63%) and medulla (69%). No change was seen in the pelvis volume. Insulin administration on day 21 to untreated diabetic rats abruptly reduced kidney volume by 21%, cortex volume by 27% and medulla volume by 18%, and after 4 days all three parameters were similar to those seen in non-diabetic controls. In conclusion, the present study has demonstrated the applicability of magnetic resonance imaging as a non-invasive measure of renal enlargement and regression in experimental diabetes in rats. In untreated hyperglycaemic diabetic rats, with manifest diabetic renal enlargement, acute regression in renal volume was observed concomitant with insulin-induced normoglycaemia.
本研究的目的是利用磁共振成像作为大鼠实验性糖尿病中肾脏增大和恢复的非侵入性测量方法。大鼠被随机分为三组：（1）非糖尿病对照动物；（2）糖尿病动物，链脲佐菌素治疗后30天未治疗；（3）链脲佐菌素给药后第21天至第30天用胰岛素治疗的糖尿病动物。从麻醉的大鼠获取磁共振图像，并在整个实验直至第30天计算肾脏、皮质、髓质和肾盂的体积。在未治疗的糖尿病动物中，糖尿病诱导后4天可见肾脏体积显著增加（P<0.05），30天后这种增加达73%，皮质（63%）和髓质（69%）有类似增加。肾盂体积未见变化。在第21天对未治疗的糖尿病大鼠给予胰岛素，使肾脏体积突然减少21%，皮质体积减少27%，髓质体积减少18%，4天后所有三个参数与非糖尿病对照相似。总之，本研究证明了磁共振成像作为大鼠实验性糖尿病中肾脏增大和恢复的非侵入性测量方法的适用性。在未治疗的高血糖糖尿病大鼠中，伴有明显的糖尿病性肾脏增大，观察到肾脏体积的急性恢复与胰岛素诱导的血糖正常相关。
