Germain S, Philippe J, Fuchs S, Lengronne A, Corvol P, Pinet F
INSERM Unit 36, Collège de France, Paris.
FEBS Lett. 1997 Apr 28;407(2):177-83. doi: 10.1016/s0014-5793(97)00332-3.
Calu-6 cells were characterized for studying the transcriptional regulation of the human renin gene. Analysis of cis-acting elements of the renin promoter showed the highest activity within the first 582 bp in serum-free conditions and of the 892 bp in the presence of serum. cAMP activates renin mRNA synthesis parallel to renin production (20-fold increase) as well renin promoter activity (2-fold). cAMP response element and the (-77 to -67) element are both necessary for activation of the renin promoter but do not act independently. Functional analysis of Intron A revealed the presence of a silencer specific to renin-producing cells.
