Benten W P, Lieberherr M, Sekeris C E, Wunderlich F
Division of Molecular Parasitology and Centre for Biological and Medical Research, Heinrich Heine University, Düsseldorf, Germany.
FEBS Lett. 1997 Apr 28;407(2):211-4. doi: 10.1016/s0014-5793(97)00346-3.
Using the Fura-2 method we investigated a possible direct action of testosterone on cytosolic free calcium of splenic T cells isolated from female C57BL/10 mice. Testosterone at physiological concentrations of 1-10 nM induces an increase in [Ca2+]i within seconds, which is due to Ca2+ influx and not to Ca2+ release from intracellular stores. In contrast, estradiol induces both Ca2+ influx and Ca2+ release. The testosterone-induced Ca2+ influx is mediated by Ni2+-blockable channels and is not inhibited by cyproterone, a blocker of the classical androgen receptor. Ca2+ influx can also be induced by testosterone conjugated to BSA which is impermeable to the plasma membrane. These data indicate a novel mode of direct action of testosterone on T cells which is not mediated through the classical androgen receptor response, but through unconventional plasma membrane receptors.
我们使用Fura-2方法研究了睾酮对从雌性C57BL/10小鼠分离的脾T细胞胞质游离钙的可能直接作用。生理浓度为1 - 10 nM的睾酮在数秒内可诱导细胞内钙离子浓度（[Ca2+]i）升高，这是由于钙离子内流而非细胞内钙库释放钙离子所致。相比之下，雌二醇可诱导钙离子内流和释放。睾酮诱导的钙离子内流由镍离子可阻断的通道介导，且不受经典雄激素受体阻滞剂环丙孕酮的抑制。与牛血清白蛋白（BSA）结合的睾酮（其不能透过质膜）也可诱导钙离子内流。这些数据表明睾酮对T细胞具有一种新的直接作用模式，该作用并非通过经典雄激素受体反应介导，而是通过非传统的质膜受体介导。
