Beilharz T, Beddoe T, Landl K, Cartwright P, Lithgow T
School of Biochemistry, La Trobe University, Bundoora, Australia.
FEBS Lett. 1997 Apr 28;407(2):220-4. doi: 10.1016/s0014-5793(97)00349-9.
Yeast cells harboring mft1 mutations are compromised in mitochondrial protein targeting, and Mft1p has previously been identified as a ribosomal protein. However, two genes, PLC2 and YML062C, are present in the MFT1 locus, and we show that mft1 mutant cells are compromised in the function of the cytosolic protein encoded by YML062C. The ribosomal protein (YS3a) is actually encoded by the tightly linked PLC2 gene, and does not play a role in targeting proteins to the mitochondria.
携带mft1突变的酵母细胞在线粒体蛋白质靶向方面存在缺陷，并且Mft1p先前已被鉴定为一种核糖体蛋白。然而，MFT1基因座中存在两个基因PLC2和YML062C，我们发现mft1突变细胞中由YML062C编码的胞质蛋白功能存在缺陷。核糖体蛋白（YS3a）实际上由紧密连锁的PLC2基因编码，在将蛋白质靶向线粒体的过程中不起作用。
