腹膜液细胞块材料革兰氏染色在细菌性腹膜炎中的价值。
Value of Gram's stain of cell block material from peritoneal fluid in bacterial peritonitis.
作者信息
Renshaw A A, Doolittle M H
机构信息
Department of Pathology, Brigham & Women's Hospital, Boston, Massachusetts 02115, USA.
出版信息
Acta Cytol. 1997 May-Jun;41(3):755-8. doi: 10.1159/000332699.
OBJECTIVE
To determine the value of Gram's stain of cell blocks of peritoneal fluid for the diagnosis of bacterial peritonitis.
STUDY DESIGN
The results of Gram's stain of 12 cases of culture-positive, frank bacterial peritonitis (FBP) and 11 cases of culture-positive spontaneous bacterial peritonitis (SBP) were compared with 6 cases of culture-negative frank sterile peritonitis and 21 cases of culture-negative spontaneous sterile peritonitis.
RESULTS
For both FBP and SBP, Gram's stain had low sensitivity (8% and 9%). Overall, the positive predictive value of Gram's stain for peritonitis was 33%. Hematoxylin-eosin (HE) was just as sensitive but had a positive predictive value of 100%.
CONCLUSION
In cell block material of peritoneal fluid, Gram's stain is of little value except to further characterize organisms seen on HE.
目的
确定腹腔积液细胞块革兰氏染色对细菌性腹膜炎诊断的价值。
研究设计
将12例培养阳性的急性细菌性腹膜炎（FBP）和11例培养阳性的自发性细菌性腹膜炎（SBP）的革兰氏染色结果，与6例培养阴性的急性无菌性腹膜炎和21例培养阴性的自发性无菌性腹膜炎进行比较。
结果
对于FBP和SBP，革兰氏染色敏感性均较低（分别为8%和9%）。总体而言，革兰氏染色对腹膜炎的阳性预测值为33%。苏木精-伊红（HE）染色敏感性与之相同，但阳性预测值为100%。
结论
在腹腔积液细胞块材料中，革兰氏染色除了能进一步鉴定HE染色所见微生物外，价值不大。
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