Svendsen P C, Yee H A, Winkfein R J, van de Sande J H
Department of Medical Biochemistry, University of Calgary, Health Sciences Centre, Alberta, Canada.
Gene. 1997 Apr 21;189(2):175-81. doi: 10.1016/s0378-1119(96)00797-4.
Uracil-DNA glycosylase (UDG) is the enzyme responsible for the first step in the base-excision repair pathway that specifically removes uracil from DNA. Here we report the isolation of the cDNA and genomic clones for the mouse uracil-DNA glycosylase gene (ung) homologous to the major placental uracil-DNA glycosylase gene (UNG) of humans. The complete characterization of the genomic organization of the mouse uracil-DNA glycosylase gene shows that the entire mRNA coding region for the 1.83-kb cDNA of the mouse ung gene is contained in an 8.2-kb SstI genomic fragment which includes six exons and five introns. The cDNA encodes a predicted uracil-DNA glycosylase (UDG) protein of 295 amino acids (33 kDa) that is highly similar to a group of UDGs that have been isolated from a wide variety of organisms. The mouse ung gene has been mapped to mouse chromosome 5 using fluorescence in situ hybridization (FISH).
尿嘧啶-DNA糖基化酶（UDG）是碱基切除修复途径第一步的负责酶，该途径能特异性地从DNA中去除尿嘧啶。在此，我们报告了与人类主要胎盘尿嘧啶-DNA糖基化酶基因（UNG）同源的小鼠尿嘧啶-DNA糖基化酶基因（ung）的cDNA和基因组克隆的分离。对小鼠尿嘧啶-DNA糖基化酶基因的基因组组织进行的完整表征表明，小鼠ung基因1.83 kb cDNA的整个mRNA编码区包含在一个8.2 kb的SstI基因组片段中，该片段包括6个外显子和5个内含子。该cDNA编码一个预测的由295个氨基酸（33 kDa）组成的尿嘧啶-DNA糖基化酶（UDG）蛋白，它与从多种生物体中分离出的一组UDG高度相似。利用荧光原位杂交（FISH）技术，已将小鼠ung基因定位到小鼠5号染色体上。
