Gagro A, Dasić G, Sabioncello A, Rabatić S, Reckzeh B, Havemann K, Kardum I, Jacksić B, Vitale B
Institute of Immunology, Zagreb, Croatia.
Leuk Lymphoma. 1997 Apr;25(3-4):301-11. doi: 10.3109/10428199709114169.
Whole-blood three-color immunofluorescence analysis was used to investigate the role of CD5/CD72 and CD21/CD23 receptor-ligand pair formation on B-chronic lymphocytic leukemia (B-CLL) cells as well as sCD23 and bcl-2 oncoprotein expression in disease progression and activity and total tumor mass in B-cell chronic leukemia (B-CLL) patients. Thirty-four patients with B-CLL and 19 controls were included in the study. The majority of B-cells in B-CLL patients coexpressed CD5 and CD72 as well as the CD23 antigen. Unlike B-cells in B-CLL patients, B-cells in all healthy controls tested had high expression of CD21 antigen. We identified two groups of B-CLL patients according to high (n = 20) or low levels (n = 14) of CD21 expression on CD19+CD23+ B-cells. Only in the patients with high CD21 expression, were sCD23 levels positively correlated with factors known to have prognostic significance in B-CLL (Rai stage and TTM) and could, therefore, be used as a prognostic parameter for these B-CLL patients. Bcl-2 oncoprotein expression did not differ between these patient groups. We presumed that in patients with a lower expression of CD21 antigen, the contribution of the CD21 molecule to homotypic adhesion was lacking. Further studies are necessary to determine the possible association of higher expression of the CD21 antigen with disease progression and the aggressive character of the B-CLL.
采用全血三色免疫荧光分析，研究CD5/CD72和CD21/CD23受体 - 配体对形成在B细胞慢性淋巴细胞白血病（B - CLL）细胞上的作用，以及可溶性CD23（sCD23）和bcl - 2癌蛋白表达在B细胞慢性白血病（B - CLL）患者疾病进展、活性和总肿瘤量中的作用。34例B - CLL患者和19例对照纳入本研究。B - CLL患者中的大多数B细胞共表达CD5和CD72以及CD23抗原。与B - CLL患者的B细胞不同，所有检测的健康对照中的B细胞均高表达CD21抗原。根据CD19 + CD23 + B细胞上CD21表达的高水平（n = 20）或低水平（n = 14），我们将B - CLL患者分为两组。仅在CD21高表达的患者中，sCD23水平与已知对B - CLL具有预后意义的因素（Rai分期和总肿瘤质量（TTM））呈正相关，因此可作为这些B - CLL患者的预后参数。这些患者组之间的bcl - 2癌蛋白表达无差异。我们推测，在CD21抗原表达较低的患者中，缺乏CD21分子对同型黏附的作用。需要进一步研究以确定CD21抗原的高表达与疾病进展及B - CLL侵袭性特征之间可能存在的关联。
