小鼠耳聋突变体shaker-2区域的遗传与物理定位

Genetic and physical delineation of the region of the mouse deafness mutation shaker-2.

作者信息

Wakabayashi Y, Kikkawa Y, Matsumoto Y, Shinbo T, Kosugi S, Chou D, Furuya M, Jishage K, Noda T, Yonekawa H, Kominami R

机构信息

First Department of Biochemistry, Niigata University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1997 May 8;234(1):107-10. doi: 10.1006/bbrc.1997.6595.

DOI:10.1006/bbrc.1997.6595

PMID:9168970

Abstract

A total of 951 backcross progeny have been obtained from a backcross segregating for the mouse deafness mutation, shaker-2(sh-2). Linkage analysis provides a detailed genetic map in the vicinity of sh-2 which comprises 40 backcross mice identified as recombinant within a 4 cM region. This allows construction of a contig consisting of 21 BAC clones across an approximately 700-kb region of sh-2. This covers the entire nonrecombinant region of sh-2 and is therefore useful to facilitate the identification of genes in the sh-2 region.

摘要

通过对小鼠耳聋突变体shaker-2（sh-2）进行回交分离，总共获得了951个回交后代。连锁分析提供了sh-2附近的详细遗传图谱，该图谱包含40只在4厘摩区域内被鉴定为重组体的回交小鼠。这使得构建一个由21个BAC克隆组成的重叠群成为可能，该重叠群跨越了sh-2大约700千碱基的区域。这覆盖了sh-2的整个非重组区域，因此有助于鉴定sh-2区域中的基因。