McGuire R E, Daiger S P, Green E D
Human Genetics Center, University of Texas Health Science Center, Houston 77225, USA.
Genomics. 1997 May 1;41(3):481-4. doi: 10.1006/geno.1997.4689.
ADP-ribosylation factor 5 (ARF5) is a member of the ARF gene family. The ARF proteins stimulate the in vitro ADP-ribosyltransferase activity of cholera toxin and appear to play a role in vesicular trafficking in vivo. We have mapped ARF5, one of the six known mammalian ARF genes, to a well-defined yeast artificial chromosome contig on human chromosome 7q31.3. In addition, we have isolated and sequenced an approximately 3.2-kb genomic segment that contains the entire ARF5 coding region, revealing the complete intron-exon structure of the gene. With six coding exons and five introns, the genomic structure of ARF5 is unique among the mammalian ARF genes and provides insight about the evolutionary history of this ancient gene family.
ADP核糖基化因子5（ARF5）是ARF基因家族的成员之一。ARF蛋白可刺激霍乱毒素的体外ADP核糖基转移酶活性，并且似乎在体内囊泡运输中发挥作用。我们已将六个已知的哺乳动物ARF基因之一的ARF5定位到人类染色体7q31.3上一个明确的酵母人工染色体重叠群。此外，我们分离并测序了一个约3.2kb的基因组片段，该片段包含整个ARF5编码区，揭示了该基因完整的内含子-外显子结构。ARF5具有六个编码外显子和五个内含子，其基因组结构在哺乳动物ARF基因中是独特的，并为这个古老基因家族的进化历史提供了见解。
