Ottosson A, Edvinsson L
Department of Forensic Medicine, University Hospital, Lund, Sweden.
Cephalalgia. 1997 May;17(3):166-74. doi: 10.1046/j.1468-2982.1997.1703166.x.
The aim of the present study was to examine if the neuropeptides substance P (SP), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP) can stimulate histamine release from mast cells in the dura mater and thereby play a role in cranial vasoregulation and local neurogenic inflammation. Dura mater mast cells were compared with peritoneal mast cells in the rat. Histamine was released from dura mater mast cells by compound 48/80, SP and CGRP but from peritoneal mast cells only by compound 48/80 and SP. NPY and VIP released quite small amounts of histamine from dural mast cells. The release of SP and CGRP from rat dura mater mast cells was blocked by the receptor antagonists FK888 and CGRP8-37 respectively, suggesting receptor mediated release mechanisms. None of the stimuli released histamine from human or porcine dural mast cells, possibly because the sampling procedure injures and incapacitates the cells.
本研究的目的是检验神经肽P物质（SP）、降钙素基因相关肽（CGRP）、神经肽Y（NPY）和血管活性肠肽（VIP）是否能刺激硬脑膜肥大细胞释放组胺，从而在颅血管调节和局部神经源性炎症中发挥作用。将大鼠硬脑膜肥大细胞与腹膜肥大细胞进行比较。化合物48/80、SP和CGRP可使硬脑膜肥大细胞释放组胺，但只有化合物48/80和SP能使腹膜肥大细胞释放组胺。NPY和VIP从硬脑膜肥大细胞释放的组胺量相当少。受体拮抗剂FK888和CGRP8 - 37分别阻断了大鼠硬脑膜肥大细胞释放SP和CGRP，提示存在受体介导的释放机制。这些刺激物均未使人或猪的硬脑膜肥大细胞释放组胺，可能是因为取样过程会损伤细胞并使其失去活性。
