Feldhoff A, Kikkert M, Kormelink R, Krczal G, Goldbach R, Peters D
Department of Virology, Agricultural University of Wageningen, The Netherlands.
Arch Virol. 1997;142(4):781-93. doi: 10.1007/s007050050118.
A new purification procedure for the tospoviruses of serogroups II and III was developed. SDS-polyacrylamide gel electrophoresis of purified tomato spotted wilt virus (TSWV; serogroup I), groundnut ringspot virus (GRSV: serogroup II), tomato chlorotic spot virus (TCSV; serogroup II) and impatiens necrotic spot virus (INSV; serogroup III) showed that the glycoprotein G2 of serogroup II members differs significantly in size from that of the serogroup I virus. Western immunoblot analysis using polyclonal antisera produced against purified glycoproteins TSWV-G1 and G2 as well as peptides of hydrophilic sequences of TSWV-G1 and G2 expressed in Escherichia coli demonstrated a higher homology amongst G1 of different serogroups than for G2. These results are supported by comparing the glycoprotein gene sequences of different serogroups.
已开发出一种针对血清群II和III的番茄斑萎病毒属病毒的新纯化程序。对纯化的番茄斑萎病毒（TSWV；血清群I）、花生环斑病毒（GRSV：血清群II）、番茄褪绿斑病毒（TCSV；血清群II）和凤仙花坏死斑病毒（INSV；血清群III）进行SDS聚丙烯酰胺凝胶电泳分析，结果表明血清群II成员的糖蛋白G2在大小上与血清群I病毒的糖蛋白G2有显著差异。使用针对纯化的TSWV - G1和G2糖蛋白以及在大肠杆菌中表达的TSWV - G1和G2亲水性序列肽段产生的多克隆抗血清进行的Western免疫印迹分析表明，不同血清群的G1之间的同源性高于G2。通过比较不同血清群的糖蛋白基因序列，这些结果得到了支持。
