利用针对番茄斑萎病毒主要结构蛋白产生的多克隆抗体对番茄斑萎病毒属病毒进行血清学比较。

Serological comparison of tospoviruses with polyclonal antibodies produced against the main structural proteins of tomato spotted wilt virus.

作者信息

Feldhoff A, Kikkert M, Kormelink R, Krczal G, Goldbach R, Peters D

机构信息

Department of Virology, Agricultural University of Wageningen, The Netherlands.

出版信息

Arch Virol. 1997;142(4):781-93. doi: 10.1007/s007050050118.

DOI:10.1007/s007050050118

PMID:9170504

Abstract

A new purification procedure for the tospoviruses of serogroups II and III was developed. SDS-polyacrylamide gel electrophoresis of purified tomato spotted wilt virus (TSWV; serogroup I), groundnut ringspot virus (GRSV: serogroup II), tomato chlorotic spot virus (TCSV; serogroup II) and impatiens necrotic spot virus (INSV; serogroup III) showed that the glycoprotein G2 of serogroup II members differs significantly in size from that of the serogroup I virus. Western immunoblot analysis using polyclonal antisera produced against purified glycoproteins TSWV-G1 and G2 as well as peptides of hydrophilic sequences of TSWV-G1 and G2 expressed in Escherichia coli demonstrated a higher homology amongst G1 of different serogroups than for G2. These results are supported by comparing the glycoprotein gene sequences of different serogroups.

摘要

已开发出一种针对血清群II和III的番茄斑萎病毒属病毒的新纯化程序。对纯化的番茄斑萎病毒（TSWV；血清群I）、花生环斑病毒（GRSV：血清群II）、番茄褪绿斑病毒（TCSV；血清群II）和凤仙花坏死斑病毒（INSV；血清群III）进行SDS聚丙烯酰胺凝胶电泳分析，结果表明血清群II成员的糖蛋白G2在大小上与血清群I病毒的糖蛋白G2有显著差异。使用针对纯化的TSWV - G1和G2糖蛋白以及在大肠杆菌中表达的TSWV - G1和G2亲水性序列肽段产生的多克隆抗血清进行的Western免疫印迹分析表明，不同血清群的G1之间的同源性高于G2。通过比较不同血清群的糖蛋白基因序列，这些结果得到了支持。